In <edbeaty-240794192301 at koniskyj3.life.uiuc.edu> edbeaty at uxa.cso.uiuc.edu (ed beaty) writes:
>Hi,
>Our lab is purifying a number of proteins for N-terminal sequencing and
>other applications. We usually silver-stain our gels to make sure there
>are no contaminating proteins which could interfere with our sequencing.
>The problem is that our MW standards (from Sigma) are designed for
>Coomassie Blue staining, and give many strong "mystery bands" when we
>silver stain them. I assume this is because the proteins used to make the
>standards aren't too pure to begin with. We've tried diluting the
>standards more to make the mystery bands fainter, but some of these bands
>are nearly as strong as the expected bands.
>Does anyone know of a cleaner set of MW standards that are commercially
>available and give (reasonably) clean bands on a silver stained gel (i.e.
>good enough to send to a reviewer without having to say, "okay, ignore
>these twelve bands; that's BSA, that's lysozyme...or is it THAT band...")
>Thanks fer yer help.
>Ed Beaty
>edbeaty at uxa.cso.uiuc.edu
Try the silver stain standards in the Sigma catalog (p. 1751, 1994
catalog). I have not personally used these standards but I know that
you can not use normal standards with silver stain and expect nice
results.
If anyone knows of other sources for silver stain markers please let
me know. I am in search of markers suitable for silver stain in a low
molecular weight range (2500 - 10000). Post to this group or send me
e-mail.
Alan Kaiser
alkaiser at eden.rutgers.edu
