In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
wrote:
> Has anyone tried using o-pthalaldehyde to fluorescently label proteins
> and then run them on a gel?
>> I've heard that the fluorescent conjugate is short lived (approx 2 hr.s)
> but I haven't gotten a good explanation about what the nature of the
> instability is. Is it photo-bleaching or some other sort of breakdown.
>>> Steve Striepeke
>sstriepk at crl.com
Could someone post a reference describing this conjugation? Sounds
interesting, but I'm too lazy to do a search. Thanks.
--
"A well-done experiment gives the same sense of satisfaction that a
composer feels after composing a sonata." --Hilary Koprowsky
Steve Rogers
Dept. of Cell & Structural Biology
Univ. of Illinois @ C-U
steve_rogers at qms1.life.uiuc.edu