The labeling reaction involves the formation of a fluorescent structure
by conjugating the o-pthalaldehye with a primary amine on a protein and
the thiol of mercaptoethanol in the presence of sodium thiocyanate,
ethyl acetate, methanol, and Brij-35 in water.
Pierce sells it as Fluoraldehyde Protein\Peptide assay reagent
Steve
sstriepk at crl.com
Steve Rogers
(steve_rogers at qms1.life.uiuc.edu) wrote:
: In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
: wrote:
: > Has anyone tried using o-pthalaldehyde to fluorescently label proteins
: > and then run them on a gel?
: >
: > I've heard that the fluorescent conjugate is short lived (approx 2 hr.s)
: > but I haven't gotten a good explanation about what the nature of the
: > instability is. Is it photo-bleaching or some other sort of breakdown.
: >
: >
: > Steve Striepeke
: > sstriepk at crl.com
: Could someone post a reference describing this conjugation? Sounds
: interesting, but I'm too lazy to do a search. Thanks.
: --
: "A well-done experiment gives the same sense of satisfaction that a
: composer feels after composing a sonata." --Hilary Koprowsky
: Steve Rogers
: Dept. of Cell & Structural Biology
: Univ. of Illinois @ C-U
:steve_rogers at qms1.life.uiuc.edu
