In article <edbeaty-240794192301 at koniskyj3.life.uiuc.edu>, edbeaty at uxa.cso.uiuc.edu (ed beaty) says:
>>Hi,
>>Our lab is purifying a number of proteins for N-terminal sequencing and
>other applications. We usually silver-stain our gels to make sure there
>are no contaminating proteins which could interfere with our sequencing.
>The problem is that our MW standards (from Sigma) are designed for
>Coomassie Blue staining, and give many strong "mystery bands" when we
>silver stain them. I assume this is because the proteins used to make the
>standards aren't too pure to begin with. We've tried diluting the
>standards more to make the mystery bands fainter, but some of these bands
>are nearly as strong as the expected bands.
>>>Does anyone know of a cleaner set of MW standards that are commercially
>available and give (reasonably) clean bands on a silver stained gel (i.e.
>good enough to send to a reviewer without having to say, "okay, ignore
>these twelve bands; that's BSA, that's lysozyme...or is it THAT band...")
>>Thanks fer yer help.
>>Ed Beaty
>edbeaty at uxa.cso.uiuc.edu
Do you get a sequence after silver staining? Some time ago we tried to sequence
something that had been gold stained and found the stain completely prevented
sequencing. I then assumed that silver stains would give a similar effect but have
never tried the experiment.
Any info. would be appreciated.
