long term protein storage

Song Tan tan at aeolus.vmsmail.ethz.ch
Thu Jul 21 05:34:17 EST 1994

In article <30k6g4$3r1 at news.iastate.edu>, bipin at iastate.edu (Bipin K
Dalmia) wrote:
[comments about purifying protein and wanting to store for long time deleted]
> 50 mM tris-hcl pH 8.0
> 125 mM nacl
> 0.5 mM PMSF
> 0.5 mM EDTA
> 1 mM DTT
> 0.05 % NaN3
> 1 % triton x-100
> 50 % glycerol
> protein concentration= 1.1 mg/ml
> any suggestions/comments?
> bip

Storage buffer looks fine.  For long term storage, I would suggest
aliquoting into Eppendorf tubes, flash freezing in liquid nitrogen and
storing at -80C.  For shorter term (i.e. weeks), you may find it
convenient to have stuff at -20C.  Since you've got 50% glycerol in the
storage buffer, you can simply take out what you need without having to
thaw the sample.

Hope this helps.

Song Tan
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email:  tan at aeolus.vmsmail.ethz.ch

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