i've finally managed to purify enough of a protein expressed as a GST
fusion protein and cleaved with thrombin. (the protein is greater
than 99% pure on a silver-stained sds-gel). now for long term storage
(the protein will be used as a standard in quantitative ELISAs) i've put
the protein in the following concoction. the protein seems to be pretty
stable (at least soluble, i'm not too concerned about activity) during
the purification steps.
50 mM tris-hcl pH 8.0
125 mM nacl
0.5 mM PMSF
0.5 mM EDTA
1 mM DTT
0.05 % NaN3
1 % triton x-100
50 % glycerol
protein concentration= 1.1 mg/ml
any suggestions/comments?
bip
--
bipin k. dalmia the other night i was lying on my bed, looking
bipin at iastate.edu up at the beautiful stars, and i said to myself,
n2.bkd at isumvs.iastate.edu 'where the F*CK is my ROOF !!'
--
