We're trying to express a protein in minimal media, and I have no idea how long this usually takes. The problem is to keep all sources of nitrogen (but 15N ammonium chloride) out of the mix. Should this expression be takin 36 hours to reach OD600nm=0.5 ?
Has anyone tried to do this? I'm curious to see if the levels of expression are similar to that obtained in LB (rich) media.
Thanks,
Chris Larsen
