Mitochondrial protein help

Nora Plesofsky-Vig npv at MANI.CBS.UMN.EDU
Thu Dec 1 20:24:28 EST 1994

Our laboratory has isolated and continues to study the cytochrome c  
oxidase of Neurospora crassa. It is our experience that subunit 1 of  
the oxidase aggregates when it is boiled in SDS sample buffer in  
preparation for electrophoresis. Instead, we add SDS sample buffer to  
the proteins, leave at room temperature for 1 hr, followed by 4oC  
overnight. You might try this protocol before electrophoresis to see  
if the "contaminant" disappears. Good luck.

Nora Plesofsky-Vig

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