Fluorescent protein labeling

Joe Mack mack at ncifcrf.gov
Mon Aug 1 09:52:22 EST 1994

In article <steve_rogers-270794162122 at gellab.life.uiuc.edu> steve_rogers at qms1.life.uiuc.edu (Steve Rogers) writes:
>In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
>> Has anyone tried using o-pthalaldehyde to fluorescently label proteins 
>> and then run them on a gel?
>> I've heard that the fluorescent conjugate is short lived (approx 2 hr.s) 
>> but I haven't gotten a good explanation about what the nature of the 
>> instability is. Is it photo-bleaching or some other sort of breakdown.

It's my understanding that the formation of the complex is not very
strong and requires dehydration. In dilute solution the o-PTH will
come off.

	Joe Mack
	mack at ncifcrf.gov

>> Steve Striepeke
>> sstriepk at crl.com
>Could someone post a reference describing this conjugation?  Sounds
>interesting, but I'm too lazy to do a search.  Thanks.
>"A well-done experiment gives the same sense of satisfaction that a
>composer feels after composing a sonata."  --Hilary Koprowsky
>Steve Rogers
>Dept. of Cell & Structural Biology
>Univ. of Illinois @ C-U
>steve_rogers at qms1.life.uiuc.edu

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