In article <steve_rogers-270794162122 at gellab.life.uiuc.edu> steve_rogers at qms1.life.uiuc.edu (Steve Rogers) writes:
>In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
>wrote:
>>> Has anyone tried using o-pthalaldehyde to fluorescently label proteins
>> and then run them on a gel?
>>>> I've heard that the fluorescent conjugate is short lived (approx 2 hr.s)
>> but I haven't gotten a good explanation about what the nature of the
>> instability is. Is it photo-bleaching or some other sort of breakdown.
It's my understanding that the formation of the complex is not very
strong and requires dehydration. In dilute solution the o-PTH will
come off.
Joe Mack
mack at ncifcrf.gov
>>>>>> Steve Striepeke
>>sstriepk at crl.com>>>Could someone post a reference describing this conjugation? Sounds
>interesting, but I'm too lazy to do a search. Thanks.
>>--
>"A well-done experiment gives the same sense of satisfaction that a
>composer feels after composing a sonata." --Hilary Koprowsky
>>Steve Rogers
>Dept. of Cell & Structural Biology
>Univ. of Illinois @ C-U
>steve_rogers at qms1.life.uiuc.edu
