Posting on behalf of M. G. Smyth & P. Gunning - D. Ricke
From: SMTP%"gunnip at essex.ac.uk" 5-NOV-1993 08:02:52.46
Subj: Assistance required
From: P Gunning <gunnip at essex.ac.uk>
Subject: Electroelution & Silver-Staining
Q1. Can anyone tell me how to electroelute a protein ( MW 146 kDa,) from a urea
gel ( 8M?) I've tried the usual electroelution methods with no success.
Q2. The quickest silver staining method I know is the one by Heukeshoven and
Dernick, 1985, electrophoresis 6:103.) I did try a rapid stain from Biorad but
this method was not as good as the one by H&D. Can anyone recommend
alternatives?
Please reply to : smyth at afrc.ac.uk, because I don't have access to USENET/BIONET
Thanks,
Martin G. Smyth
________________________________________________________________________________
Martin G. Smyth
AFRC Institute for Animal Health
Compton Laboratory
Berkshire RG16 0NN
UK
Ph: +44 635 578411 ext 541 ( Laboratory, Mon-Fri 0900 to 1700 GMT )
+44 635 578888 ( Laboratory, outside above hours )
+44 635 578137 ( Home )
e-mail: smyth at afrc.ac.uk
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Many thanks,
Paul Gunning
Physics Department
University of Essex
Colchester CO4 3SQ
