In article <2d2pt0$cgp at mserv1.dl.ac.uk>, MJ Duggan <sgex400 at sghms.lon.ac.uk> writes:
>Dear Netters,
>>Has anybody out there used Triton-X-114 for the phase separation of
>membrane proteins
?
Michael, I have not, but dusting off my proteins-detergent file I found the
following which may be helpful:
"Selective release of the Treponema pallidum outer membrane and
associated polypeptides with Triton X114"
Cunningham et al., J. Bact. 170: 5789, 1988
- they used X114 to release proteins which were distributed between
aqueous and hydrophobic phases.
In my lab we have used Tween 80 to "float" hydrophobic peptides that
associate with it when it flocculates into a surface pellicle in the
presence of ammonium sulfate:
"A highly efficient second-step concentration technique for
bacteriophages and enteric viruses using ammonium sulfate and
Tween 80"
Armon et al., Can. J. Microbiol. 34:651, 1988
-Peter Muriana
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* Peter M. Muriana, Ph.D. Phone = (317)-494-8284 *
* Dept. of Food Science FAX = (317)-494-7953 *
* Purdue University E-mail = muriana at aclcb.purdue.edu *
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