Hello,
I have a second question that I wanted to keep separate form the other one
for clarity purpose :-)).
How do you think a metabolic pathway handles the over-expression of one of
the enzyme along the way to keep the flux through the pathway constant ?
Many enzymes have been over-expressed. The purpose might have been just
enzyme purification, or trying to increase the flux through the pathway.
Quite often, the flux did not increase, and the conclusion was that a step
RbeforeS is the rate limiting step (see other post about that). But what
happens to the substrate(s)/product(s) of this enzyme that has been
over-expressed ? Are they any experimental evidences of how it is done
inside the cell ?
Pascal Hua pjh3142 at rigel.tamu.edu
Biochemistry & Biophysics
Texas A&M University.
Cross-posted to:
bionet.biology.computational
bionet.cellbiol
bionet.general
bionet.info-theory
bionet.metabolic-reg
bionet.molbio.proteins
