In <David_Nunn-061193122507 at tsikar1.life.uiuc.edu> David_Nunn at qms1.life.uiuc.edu (David Nunn) writes:
> I've got either a bum strain of EC LE30 (mutD) or it just isn't doing the
>trick (mutagenesis-wise). I've been good and kept it on minimal media-only
>one transfer since receiving it-but it still is not working. Maybe just
>need a new strain?
In minimal media, mutD can increase the background mutation frequency some
500-fold, but in rich media (i.e., LB) this increase is something like
50,000-fold. Are you doing your mutagenesis in rich media?
see Schaaper, 1988 (PNAS 85:8126); Schaaper and Radman (1989) EMBO J. 8:3511
Does this help? Hope so.
Mike
--
Michael Horsfall Ph.D. (( mjhf at troi.cc.rochester.edu
Department of Biophysics )) Phone:(716)-275-8703 (Lab)
University of Rochester 5'-GCAAGTCGGAG-3' Fax:(716)-275-6007(Dept)
