Does anyone have a favorite mutator strain of E. coli or mutator plasmid
that they have used successfully for random mutagenesis?
I'd prefer a strain or compatible plasmid in which I could mutagenize
sequences cloned in a broad host range plasmid (IncP1) and then be able to
mobilize directly out of the strain into Pseudomonas (EC strain must be
Tc-sensitive and auxotrophic)
I've got either a bum strain of EC LE30 (mutD) or it just isn't doing the
trick (mutagenesis-wise). I've been good and kept it on minimal media-only
one transfer since receiving it-but it still is not working. Maybe just
need a new strain?
Any suggestions?
David Nunn
Department of Microbiology
University of Illinois
Urbana, IL 61801
(217) 333-6131
