Dear SCIENTISTS,
I am isolating the periplasmic fraction of E.coli for release
of an enzyme. Am presently usind osmotic shock treatment( sucrose shock)
as well as combinee treatment with lysozyme. Both the methods are leading
to the lysis of the cells without miantenance of intact spheroplasts.What
could be the reason? Kindly suggest another method. Thank you.
NITIKA THAPAR.
