I have recently cloned a c-terminally processed protein and am in
the process of analysing both the precursor molecule and the processed
product. I am wondering if anyone out there could assist me by sending
me a protocol for c-terminal analysis of a peptide. My searches have
lead me to relatively "old" protocols from the early 80s and I am wondering
if there has been any recent advances in this area that I may have overlooked.
The reason I ask is because the results obtained from the use of the protocol
I have (exopeptidase, time point cleavage) gives rather dirty results that
are nearly unusable beyond two amino acids.
Any help in this area would be greatly appreciated.
(ez027854 at hamlet.ucdavis.edu)