I am trying to ascertain the subunit structure of E. coli exonuclease VII,
which is composed of 52 and 9 kDa subunits in an approximate 1:4 ratio. I
would like to try glutaraldehyde crosslinking and would appreciate any
feedback on methods, protein concentration, NaBH4 quenching,
Dizzy Dan ddiaz at cwru.bitnetbl275 at cleveland.freenet.edu
Dept. Biochemistry & Wine Tasting CWRU School of Medicine & Sushi
"I usually like to live in the past, but my time machine is broken"