Subject: Time:7:59 PM
OFFICE MEMO competitive inhibition Date:11/17/92
to the net:
i am looking for a reference that will help in designing an gel mobility shift
experiment that will allow me to calculate the equilibrium binding constant of
a ligand (dna binding protein) for a competitor (different DNA polymers)
knowing the the eq. binding constant for a high affinity site with constrained
sequence. i know im looking at around 10 -8 to 10 -9 for the high affinity
site, and the competitors will be within an order of magnitude, or two at the
most.
sincerely,
molecular biologist with a cause,
dan
