New cAMP-dependent Protein Kinase Coordinates

Daniel R. Knighton dlk at chem.ucsd.edu
Fri Dec 4 18:24:12 EST 1992

To possibly save time or minimize lost time for the potentially many
protein kinase researchers basing experiments on coordinates of the so far
sole published protein kinase crystal structure, as a representative
of the group that solved the structure, I make the following announcement
and request:

The 2.7-Angstrom X-ray crystallographic model of the recombinant mouse
cAMP-dependent protein kinase catalytic subunit complexed with the
peptide inhibitor PKI(5-24) has been revised to correct the enzyme
sequence correspondence to electron density (sequence register) for
residues 55-64 and 308-339. The first segment is part of the conserved
protein kinase catalytic domain; the second is not.
The corrections do not change the peptide backbone trace and mostly
comprise displacement of the sequence one residue to the carboxy terminus
along the backbone path in the specified regions.

Therefore, if you are currently using coordinates from PDB entry 1CPK or
coordinates obtained from UCSD before October, 1992, please get for yourself,
or for your colleagues who may not see this post, the corrected
coordinates that are now available by anonymous ftp from the Brookhaven
Protein Data Bank prerelease directory.  The prerelease file is pdb2cpk.ent
on pdb.pdb.bnl.gov ( This file will become entry 2CPK upon
distribution of the regular January 15, 1993, PDB release.

If you are not able to get the file from pdb.pdb.bnl.gov for whatever reason,
please by all means contact me, and I will e-mail the file to you.

Thank you.

Daniel Knighton

                               Daniel R. Knighton         
                           Internet: dlk at chem.ucsd.edu
      BITNET:   dknighto at UCSD                       FAX: 001-619-534-8193     
Mail Code 0654, UC San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0654, U.S.A.

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