Subject: Time:4:55 PM
OFFICE MEMO stabilizing protein-DNA interactions Date:8/14/92
fellow scientists,
i would like some help in choosing a wash buffer for a filter binding assay of
free/bound dna probes. washing leads to dissociation of the complex and a
deflation of the fraction bound at a given time point. are there any low ionic
strength solutions which could be used to wash free probe, but favor
association of bound complexes??
