stabilizing protein-DNA int

Dan Szymanski Dan_Szymanski at QMS1.LIFE.UIUC.EDU
Fri Aug 14 17:57:50 EST 1992

                       Subject:                               Time:4:55 PM
  OFFICE MEMO          stabilizing protein-DNA interactions   Date:8/14/92
fellow scientists,
i would like some help in choosing a wash buffer for a filter binding assay of
free/bound dna probes.  washing leads to dissociation of the complex and a
deflation of the fraction bound at a given time point.  are there any low ionic
strength solutions which could be used to wash free probe, but favor
association of bound complexes??

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