hello
I need some advice...I've been trying to crystallize nuclease P1 from
pennecilium citrinum for some time now...with poor results. The structure
was done{beautiful work might I add} by ARmin LAHM at EMBL. I've followed
his recipies to the letter but have had no good results......any advice
you could offer would help.I'm curently using a hganging drop
and a sitting drop setup 30% saturated amm.sulfate, 5mM ZnCl against
65% saturated amm. sulfate.
Also..where can i get the primary sequence for phoshpholipase C as well
as the refined structural coordinates? references?
thanks in advance
joe falcone
v098p82f at ubvmsb.cc.buffalo.edu
