Networkers,
Could I have some advice on Protease inhibitors? I am just about to start
using them as part of my extraction buffer. The amounts I need to buy are
quite small and for every extraction even less.
The question I would like to ask is: Is it possible to make up the
solution in the vial the chemical comes in and freeze aliquots? Often when
only small amounts of a chemical are obtained a fraction always seems to be
left in the lid/sides/bottom of the vial! and quite alot is "lost".
The chemicals I intend to use are (1) Aprotinin (2) Leupeptin (3) Pepstatin
If anyone has any alternatives/recommendations on the use of these chemicals
I would be more than grateful.
Yours
Andrew Johnston.
Andrew M. Johnston, E-Mail: BS04 at PRIMEB.DUNDEE.AC.UK
Biological Sciences, Tel No: 0382-23181 Ext 4282
University of Dundee,
Dundee
Scotland
DD1 4HN
