Thanks to MSZ at XRAY.BMC.UU.SE (Michael Szardenings, Uppsala), I have got the
address of the editor of the BRENDA database,
SCHOMBURG at VENUS.GBF-BRAUNSCHWEIG.DBP.DE. He is on vacation for a few weeks
(thanks to David Lincoln for this information), so I do not expect an answer
immediately. However, Marc Roussel (mroussel at alchemy.chem.utoronto.ca) wanted
to know what was in the book, so I typed in the names of the contents.
The facts they include are nicely broken into sections for each enzyme:
1 NOMENCLATURE
EC number
systematic name
recommended name
synonymes
cas reg no
2 REACTION AND SPECIFICITY
catalysed reaction
reaction type
natural substrates
substrate spectrum
product spectrum
inhibitor(s)
cofactor(s)/prostethic group(s)
metal compounds/salts
turnover number
specific activity
Km-value
pH-optimum
pH-range
temperature optimum
temperature range
3 ENZYME STRUCTURE
molecular weight
subunits
glycoprotein/lipoprotein
4 ISOLATION/PREPARATION
source organism
source tissue
localisation in srouce
purification
crystalization
cloned
renatured
5 STABILITY
pH
temperature
oxiddation
organic solvent
general stability information
storage
6 CROSSREFERENCES TO STRUCTURE DATABANKS
PIR/MIPS code
Brookhaven code
7 LITERATURE REFERENCES
I may have mis-spelled or missed one, but there is the juicy list! It sure is
comprehensive. It seems to be organized by EC number, but in the electronic
database one should be able to do other things I imagine. More later on when
Dr. Schomburg returns from vacation.
Tom Schneider
National Cancer Institute
Laboratory of Mathematical Biology
Frederick, Maryland 21702-1201
toms at ncifcrf.gov
