Does anyone have any experience with proteins that migrate significantly slower
by SDS-PAGE than their DNA sequence predicts? I have a 22.3 Kd ORF that yields
a 35 Kd polypeptide on gels. I would like to know if someone has similar
experience or some possible explanation(s).
Jim Gordon
University of Western Ontario,
Ontario, Canada
email: (care of) T.LINN at uwovax.uwo.ca
