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[Plant-biology] nos promoter in agro

sfkrasny from ncsu.edu via plantbio%40net.bio.net (by sfkrasny from ncsu.edu)
Wed Nov 28 10:53:49 EST 2007


Recently the construct was made were binary vector has spec resistance for
selection of E. coli containing this plasmid. Does anybody know if the nos
promoter that is driving nptII (on T-DNA) is active in Agrobacterium? In
our previous similar constructs nptII was driven by 35S not by nos
promoter and Agro could be selected with kanamycin as well (100 mg/ml). I
have read some references that nos promoter would be active but Agro could
grow with kanamycin  at lower concentration. Any comments or literature
reference?

Thanks



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