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quick and dirty total RNA quantitation from Cyanobacteria

giordano at univpm.it giordano at univpm.it
Tue May 6 11:32:36 EST 2003


I am looking for a quick and dirty way to get an estimate of total RNA extracted from the cyanobacterium Phormidium autumnale. The RNA has been extracted with a standard Phenol-Chloroform procedure and it is now frozen in DEPC-trated water. 

The purpose is just to get an estimate of total mRNA levels in P. subjected to (drastically) different light treatments.

How can I quantify this RNA with minimal interference from DNA or free bases, without having to go through complex image analysis or purification procedures?

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