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monocot transformation

Timothy P. Beary biol-tpb at nich-nsunet.nich.edu
Tue Feb 16 18:54:54 EST 1999

I am about to begin transformation of sugarcane embryonic
calli using microparticle bombardment (1um gold beads).
What are the latest thoughts as to whether or not my
plasmid DNA need be linearized?

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