We seek for a post-doc to participate in a project entitled "Transcript
profiling during ectomycorrhiza development". The objective of this
research program is to sequence and analyse genes expressed during the
development of ectomycorrhiza between a fungus and a host tree.
Mycorrhiza is found on a vast majority of all plant species including
many important forest trees and crop plants. Mycorrhizal fungi improve
the growth of the host plants by facilitating the nutrient uptake from
the soil. cDNA libraries of transcripts expressed during the symbiosis
will be constructed by differential screening and subtractive
hybridization of mRNA isolated from ectomycorrhiza, non-infected roots
and free-living hyphae of the fungus, respectively. The uniquely
expressed clones will be sequenced from either ends. Comparison of the
coding capacity of the expressed sequence tags (ESTs) with sequences in
data bases will result in the assignment of putative functions to a part
of these ESTs. To further investigate the function of the EST clones,
the temporal and spatial patterns of expression of some of the
corresponding transcripts will be examined in more detail during the
development of the ectomycorrhiza, also including when the plant and
fungus are growing in a soil system.
The candidate will work mainly at the Department of Microbial Ecology in
Lund, Sweden. Experiences in making cDNA libraries or analyzing mRNA
from fungi or plants are requested. Further informations and
applications should be sent to Dr. Anders Tunlid
(anders.tunlid at mbioekol.lu.se), Department of Microbial Ecology, Lund
University, Ecology Building, S-223 62 Lund. Phone +46-46-2223757, Fax
+46-46-2224158. Deadline for applications is March 15.
The project has been funded for two years by the Genome Research Program
of the Swedish Foundation for Strategic Research and is a joint project
between the Department of Microbial Ecology, Lund University, Sweden
(Anders Tunlid and Bengt Söderström) and the Department of
Biotechnology, Royal Intsitute of Technology (KTH) in Stockholm, Sweden
(Mathias Uhlen, Bertil Pettersson and Joakim Lundeberg).
Brun A., Chalot M., Finlay R.D and Söderström B. (1995). Structure and
function of the ectomycorrhizal association between Paxillus involutus
(Batsch) Fr. and Betula pendula (Roth.). I. Dynamics of mycorrhiza
formation. New Phytol. 129: 487-493.
Chalot M., Brun A., Botton B. and Söderström B. (1996). Characterization
of the general amino acid transporter from the ECM fungus Paxillus
involutus. Microbiology 142: 1749-1756.
Sterky F., Regan S., Karlsson J., Hertzberg M., Rohde A., Holmberg A.,
Amini B., Bhalerao R., Larsson M., Villarroel R., Boerjan W., Sandberg
G., Olsson O., Teeri T., Gustafsson P., Uhlén, M., Sundberg B. and
Lundeberg J. Gene discovery in the wood-forming tissues of poplar:
analysis of 5692 expressed sequence tags. 1998, Proc Natl Acad Sci., in
M. Ronaghi, M. Uhlén, and P. Nyrén (1998). PyroSequencing - A DNA
sequencing method based on real-time pyrophosphate detection. Science,