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dissolving of RNA

Frank Maiwald maiwald at mpiz-koeln.mpg.de
Thu Mar 20 09:31:55 EST 1997

Hi, all,

I have problems to dissolve RNA-pellets in DEPC-treated H2O. I deal with
barley total RNA that was extracted with phenol and precipitated with
acetic acid/ ethanol and with LiCl. Although I keep the samples at 65 C
and increased the volume several times, the pellets do not dissolve (or
very, very, very slowly).

Does anybody have any tips how to overcome this problem?

Thanks					Frank

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