I have problems to dissolve RNA-pellets in DEPC-treated H2O. I deal with
barley total RNA that was extracted with phenol and precipitated with
acetic acid/ ethanol and with LiCl. Although I keep the samples at 65 C
and increased the volume several times, the pellets do not dissolve (or
very, very, very slowly).
Does anybody have any tips how to overcome this problem?