IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

need help with root culture

DEPARTAMENTO DE MICROBIOLOGIA mi1masaj at lucano.uco.es
Tue Jun 24 07:18:53 EST 1997


Dear Tanya,

I have no worked with poplar but I have established root
cultures of some crops like tomato and alfalfa. Maybe you can
try the same protocol:

- Surface sterilise the seeds (1 min ethanol 95% + 20 min in
comercial bleach + three baths of 5 min. each in sterile
distilled water)

- Transfer the axenic seeds to flaks previously autoclaved
containing humid cotton.

- cut 1 cm of the emerging root (including the root tip) and
transfer it to 250 ml Erlenmeyer flasks containing 50 ml of
autoclaved MS medium + sucrose 3%. Incubate in an orbital
shaker 130 rpm aprox. 25† C.

- Usually the roots growth an multiply producing lateral roots
that can be cuted an subcultured in fresh media.


																		Best wishes,


																	Xabier Barandiaran





More information about the Plantbio mailing list

Send comments to us at biosci-help [At] net.bio.net