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Plant Protoplast Plating

Ben Levin benjinsl at astro.ocis.temple.edu
Thu May 30 14:00:40 EST 1996

 I was recently thinking of methods of plant propagation and wondered if 
anyone had ever used plant protoplasts.  I would imagine the procedure 
would involve vacuum-infiltrating a piece of tissue with a cellulase 
soln. and then spinning down the freed protoplasts.  The resuspended 
protoplasts could then be plated on the appropriate nutrient media and 
grown into plants or kept in broth to increase their numbers for 
subsequent plating.


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