On Wed, 05 Jun 1996 13:40:46 -0500, c670769 at showme.missouri.edu wrote:
>Someone mentioned xylem cells being mostly dead. that certainly could
>have a lot to do with your problems. It seems that also HOW you
>sterilize your equipment may also be important. If you are merely
>autoclaving your glassware, it will not kill off all the RNAses.
>Baking of glassware is essential in most RNA preps. Also in some
>cases gloves won't do it either. Some need lab coat, hair net, etc.
>>Hope some of this helps
Killing off the RNases is a myth... I use autoclaved tips, clean
glassware and DEPC-treated water for all the RNA extractions I've done
so far and I haven't had any degraded RNA yet. I believe that being
careful not to introduce new RNAses into your preparation is much more
important than worrying about the ones that are already there.
Ph.D. Student in Molecular Pharmacology
Centre de Recherche de l'Hotel-Dieu de Quebec
e-mail : jfl at rsvs.ulaval.cajfl at zone.ca