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pET vectors and low yields

Gilles VACHON vachon at bio.grenet.fr
Mon Jan 16 04:55:34 EST 1995

Dear netters,

I am having trouble in getting regular amounts of pET (19b or 11a, with ot
without insert)) in DH5alpha.
I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride prep).

The DH5alpha strain is ok since I get 500-700ug of pUC in the same conditions.

Does anyone on the net have an answer to that problem ?
I am planning to do cloning with these pETs and I bet I won't be able to
check the clonings with that low yield.

Dr. Gilles Vachon
Laboratoire de Biologie Moleculaire Vegetale
CERMO, 3eme etage
Universite J. Fourier, BP 53X
Tel: (33) 76 51 48 92
fax: (33) 76 51 43 36
e-mail: vachon at bio.grenet.fr

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