Chris Jones (Chris_Jones at nottingham.ac.uk) wrote:
: In article <3htugf$aou at emory.mathcs.emory.edu> medtjm at bimcore.emory.edu (T. J. Murphy) writes:
: >From: medtjm at bimcore.emory.edu (T. J. Murphy)
: >Subject: Bizarre Transgene Idea?
: >Date: 15 Feb 1995 22:16:47 GMT
: >Problem: I need massive quantities of a mammalian integral membrane protein.
: > Bacteria can't handle the protein.
: >Question: Could I make a transgenic tomato to overexpress my protein as a
: > source for purification? Has this been attempted? Any pro's
: > or con's?
: If yeast don't work then think about baculoviruses but I am not sure how they
: tolerate membrane proteins. I guess you know that membrane proteins are amongt
: the most difficult to express....
: Oh, Yes. Just how much is massive? and whats the coli expression problem. You
: could try a fusion in coli, fusions often lack the lethality that some prots
: show. Good luck
: Chris Jones
I recall in GEN or someother periodical seeing an advertisment for insect
cell membranes preparations containing various mammalian integral
membrane proteins. This was about a year ago.
Baculovirus may be the way to go.