Thanks for answers
Now I got some pre-modias from other explants(leaflet).
They was selected in KM 50mg/l.
and pre-modias was showen positive response about GUS assay.
as you say,
I will transfer them to KM 100mg/l selection medium.and
I also keep them on KM 50mg/l medium
thanks Dr. Jef
I read it and I am following it for transformation of
Dr. Sheila Mccormick
I appreciate your answer to my question.
Hwang Jong pil
e-mail: z942460 at axp3400.kyungpook.ac.kr