Could someone answer a question on tomato transformation?
Lycopersion Pennellii. It is easy to induce callus and shoot from explant
I succeed in making some plants from explant(leaflet) of Lycopersion
They was selected in KM 50mg/L(kanamycin) during inducing shoot, callus
but GUS expression is negative although T-DNA have a NPTII and Gus gene.
I don't experiment Southern Blot Analysis or PCR yet!!
How percent(%) do I guess about a transgenic tomato ?
Have you ever gotten the same result?
could someone explain it to me?
I would appreciate any information on this subject.
Hwang Jong Pil
e-mail: z942460 at axp3400.kyungpook.ac.kr