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DNA extractions

bio_hannan at emuvax.emich.edu bio_hannan at emuvax.emich.edu
Mon Dec 4 21:37:48 EST 1995


In article <49n9o2$7tv at mserv1.dl.ac.uk>, "Corinna. Dutton" <Corinna.Dutton at newcastle.ac.uk> writes:
> I have had problems purifying genomic DNA for southerns from potato 
> tuders and tobacco leaves.  I get problems with polysaccharide 
> contamination and low yield with CTAB based methods. Any suggestions for 
> protocols which can be carried out on an open bench would be appreciated
> 
> Corinna
You might want to try BioClean (or other glassmilk protocol) after
extraction.  I have had to use a very mild extraction (freeze/thay lysis)
followed by bioCleaning to get rid of interfering substances in Iris lacustris.

Good luck

Gary Hannan



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