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Cloning Cauliflowers

Lee Montgomerie lee at riviera.demon.co.uk
Fri Apr 28 18:20:46 EST 1995

As a non-specialist school laboratory technician I have made repeated 
attempts to clone cauliflowers.  The cloning medium I use is:

4.7g Murashige & Skoog
30g Sucrose
8g Agar
pinch of plant rooting hormone (IAA)
made up to 1 litre with distilled water
and prepared as sterile slopes in McCartney bottles

So far this method has *never* been known to suceed!  I have tried 
minor variations in heat, lighting, proportion of ingredients, 
size of cauliflower fragments, duration of experiment (at least 6 
weeks) etc, but without results.  Nobody else I know has managed it 
either, though there are apocryphal tales of sucesses.

Can anybody suggest an alternative plant/medium/technique or whatever 
which is more likely to work?

Lee Montgomerie                           EMail lee at riviera.demon.co.uk 

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