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tobacco BY2 tc cells

Ann Loraine loraine at NATURE.BERKELEY.EDU
Tue Apr 4 00:52:13 EST 1995


Dear All,

A few months ago one of you responded to my query about tobacco BY2
cells and sent me an excellent protocal for making protein extracts from
these cells.  Well, to make a long story short, now it's time to scale
up and start fractionating extracts.

Two questions:  

#1 What is the best way to disrupt a large amount of material, say, 100 mL
	packed cells?

#2 Can anyone give me references reporting purification of an activity,
	any activity!, from BY2 cells, or any plant tc line?

Thanks in advance!

Ann Loraine 



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