A few months ago one of you responded to my query about tobacco BY2
cells and sent me an excellent protocal for making protein extracts from
these cells. Well, to make a long story short, now it's time to scale
up and start fractionating extracts.
#1 What is the best way to disrupt a large amount of material, say, 100 mL
#2 Can anyone give me references reporting purification of an activity,
any activity!, from BY2 cells, or any plant tc line?
Thanks in advance!