The dam- phenotype is what is important for demethylating ClaI sites.
It can be found In JM110.
In article <2hge8q$efv at mserv1.dl.ac.uk> Egea Marcos
<marcos at techunix.technion.ac.il> writes:
>does anybody have a clue or suggestion of an EcoK r-m- strain to
>use as a host for cloning?. I have a ClaI methylated that need to
>unmethylate to cut and subclone.
>Marcos Egea Cortines