In <2hge8q$efv at mserv1.dl.ac.uk> Egea writes:
> does anybody have a clue or suggestion of an EcoK r-m- strain to
> use as a host for cloning?. I have a ClaI methylated that need to
> unmethylate to cut and subclone.
> Marcos Egea Cortines
Hi there !
I think one solution to your problem is to use the E.Coli K12 strain
HB101 which is EcoK R-M-.
By the way, I think HB101 is easy to transform, I haven't tried it myself