I have used these tubes, and they work really well. Costaer referred me to
2 refs which may help.
1) Polman, James K. and John M. Larkin. 1989. Purification of DNA from
agarose gels. Biotechnology Techniques vol.3:329-332.
2) Vogelstein, Bert. 1987. Rapid purification of DNA from agarose gels by
centrifugation through a disposable plastic column. Analytical
In a nutshell, I spin at 5000 x g (5000 rpm in an eppendorf centr.) for 10
minutes. The stuff on top is the agarose, and the liquid in the collection
tube contains the DNA. I have used this DNA in standard Maniatis type
ligations, and it works like a charm. If you need more detail, let me
On 11 Apr 1994, Egea Marcos wrote:
> Hy netters,
> I received a free sample of Spin-x centrifuge columns from Costar, but
> the dealer never sent me the protocol, can somebody out there tell me how
> to use them?
> Did you ever use it to isolate DNA from agarose + ligation afterwards?
> (Most hated reaction in the Hall of Fame).
>> Marcos Egea Cortines
> Israel Institute of Technology
> Dept. of Biology
> Technion 32000 Haifa