okellyc at megasun.BCH.UMontreal.CA (Charles J. O'Kelly) writes:
>In article <bucc0003.765611484 at gold>, bucc0003 at gold.tc.umn.edu (Paul A Bucciaglia) writes:
>|> I've been staining tobacco microspores (about 3-5 days post tetrad) with
>|> acetocarmine to follow microspore mitosis. Using the std. 1% carmine
>|> in 45% acetic acid on both fixed (ethanol/acetic acid) and unfixed antehrs
>|> gives me bright red cytoplasms that obscure any sign of a nucleus. i dug
>|> up a reference from 1937 (Maheshwari, Stain Tech. 12#2) which suggested
>|> a few drops of chloral hydrate to clear the cytoplasm. "ahh, an archaic
>|> name for HCl" i thought so i added a drop of 1N HCl. This did help some
>|> as the nucleus was just barely visible and the cytoplasm cleared somewhat.
>|> So i checked the Sigma catolog and was surprised to find that chloral
>|> hydrate is a nasty chlorinated hydrocarbon that is (was?) used to make DDT.
>Chloral hydrate was also the active ingredient in the Mickey Finn (knockout drops
>added to alcohol in all the old film noir mysteries).
>Given the above, why not relax and learn to use chloral hydrate safely?
>|> paul bucciaglia
the other problem we ran into when checking into using chloral hydrate
as an ingredient in a clearing solution was that chloral hydrate is a
controlled substance -- the permits and paperwork involved are a giant
hassel - to say nothing of the worries of storing a current "street drug"
in the lab --- we decided to work around the problem rather than go through
the federal and state controlled substance permit routine
calfor at unity.ncsu.edu