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RT-PCR from small amount of tissue

Gilles VACHON vachon at bio.grenet.fr
Tue Nov 23 11:20:14 EST 1993


Dear neters,

There has been a number of messages lately on this net dealing with PCR
directly from small amount of plant tissues.

Now what about RT-PCR from small (tiny) amount of plant tissues?
Is it possible to break or lyse the sample cells in a tube (without
crushing etc... of course) to get intact RNAs and make RT-PCR all in the
same tube (or
alternatively purify RNAs with oligo-dT magnetic beads for instance) ? 

I've heard rumours about microwaving the sample in the RT-PCR buffer for
example...



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