> To: plant-biology at net.bio.net> From: SERTAC at tr.edu.metu.cc.vm (Sertac ONDE)
> Subject: Thaumatin II: A sweet Marker Gene for Plants......
> Date: 2 Nov 93 15:29:46 GMT
> Dear netters,
> After reading a paper by Michael Witty about Thaumatin II reporter
> gene utilisation in plant genetic manipulations (which appeared in
> Methods of Enzymology 216: 441-447, 1992), one particular paragraph
> at the Concluding Remarks section (p. 447) made me curious.
> At the final paragraph, the author mentioned a phenotypic change in
> plants overexpressing the Thaumatin II under the control of CaMV35S,
> and continued to state that this phenotypic change could be used to
> detect transgenic lines or whole plants.
> Could someone inform me what this phenotypic change is all about?
> Is it a color change or more on the morphology level?
>> With my best regards,
> Dr. Sertac Onde
> Middle East Technical University, Biology Dept.
> Ankara, Turkey.
> I would also be interested in the answer to this question.
Stephen A. Pluhar
SPLUHAR at CROP.UOGUELPH.CA Dept. Crop Science U. of Guelph
Phone: 519-824-4120 Ext. 4865 Guelph, Ontario, Canada. N1G 2W1