I am trying to isolate functioning chloroplasts from brown algae Laminaria.
I succeeded in isolating chloroplasts, which looked O.K. but did not
evolve oxygen. Can anybody advice me?
I am using Trcine buffer (pH 7.5) with 0.3 M Sorbitol, 5mM EGTA,5mM EDTA,
1% BSA 5mM DTT, 5mM Ascorbate and 2% PVP as isolation medium.
Univ. of London
Campden hill Road
London W8 7AH,
b.sopko at hazel.cc.kcl.ac.uk