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Chloroplast isolation

UDBS063 at HAZEL.CC.KCL.AC.UK UDBS063 at HAZEL.CC.KCL.AC.UK
Thu Mar 4 07:12:20 EST 1993


Hi, 

I am trying to isolate functioning chloroplasts from brown algae Laminaria.
I succeeded in isolating chloroplasts, which looked O.K. but did not
evolve oxygen. Can anybody advice me?
I am using Trcine buffer (pH 7.5) with 0.3 M Sorbitol, 5mM EGTA,5mM EDTA,
1% BSA 5mM DTT, 5mM Ascorbate and 2% PVP as isolation medium.
Thanks

Bruno Sopko 
King College
Univ. of London
Campden hill Road
London W8 7AH, 

b.sopko at hazel.cc.kcl.ac.uk
net: JANET



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