"RT-PCR is one of the methods used by itself or as part of a series of
experiments which can be used to amplify and quantify RNA by producing
cDNA from mRNA. Several questions should be answered to determine if
RT-PCR is the best experimental technique to use or if other methods are
better experimental options."
The full text Alkami PCR Review of "Going Back to Look for A Message:
Reverse-Transcriptase (RT) PCR" by Dr. Laura Ruth is now available at
The article contains online links and references for the bench scientist
interested in the subject of RT-PCR. You can also find sample RT-PCR
protocols and links at http://www.alkami.com/mthdrt.htm
The Alkami Biosystems web site is devoted to offering FREE resources and
information related to the polymerase chain reaction (PCR). The site
includes resources for primer design, PCR methods, troubleshooting,
polymerases, and other PCR related topics.
Order a FREE PCR supply samples at http://www.alkami.com/pgorder.htm
Primer Design Tips - http://www.alkami.com/refprmr.htm
Primer design advice collected from a wide range of sources.
Primer Design Online - http://www.alkami.com/refdsgn.htm
Links to free online primer design tools with brief program descriptions
of each one.
PCR Troubleshooting - http://www.alkami.com/reftrbl.htm
Tips for improving your PCR specificity and efficiency, enhancers and
inhibitors, what to do for common PCR problems, and more.
PCR Methods - http://www.alkami.com/refmthd.htm
PCR method descriptions (such as RT-PCR, Hot Start PCR, In Situ, etc),
sample protocols, and related reference links.
Polymerases - http://www.alkami.com/refpoly.htm
Descriptions of commonly used thermophilic polymerases including a short
overview of characteristics (such as error rates), suggested ranges for
selected variables, and relevant reference links.
PCR Variables - http://www.alkami.com/refvrbls.htm
PCR experiment variables each with standard ranges, general information,
and ways to improve specificity, efficiency, and/or fidelity.
Visit our site at http://www.alkami.com and let us know if you
have any questions or suggestions about how we can make the Alkami
Biosystems web site an even better PCR resource for you and your
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P.O. Box 11216 Berkeley CA 94712-2216 USA