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nuclear- / mtDNA extraction

Patrick Brunner patrickcbrunner at netscape.net
Mon Nov 27 04:47:54 EST 2000


Colleagues:

I am interested in separating nuclear from mitochondrial DNA in insects for
subsequent PCR amplification of specific regions (preferably in both genomes)
and direct sequencing. 

I would like to avoid annoying gradient-centrifugation. Thus, does anybody
know of or have personal experience with a commercially available extraction
kit for this purpose?

I appreciate any help.

Best regards,

pcb


-------------------Patrick C. Brunner------------------

Swiss Federal Research Station
Schloss, Lab. 4
CH-8820 Waedenswil
Switzerland

phone: +41 (1) 783 6331    fax: +41 (1) 783 6434
e-mail: patrickcbrunner at netscape.net


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