I'm a PhD student working at the Centre for Limnology (the Netherlands).
I'm working on the molecular identification of aquatic protozoa. Using
several sequence databanks I constructed a universal eukaryotic 18S rDNA
PCR primer set. The PCR product is small enough to separate on a DGGE
gel. The method seems to be very promising in screening large numbers of
samples on eukaryotic biodiversity. Some groups of Protist have up to 4
mismatches in the forward primer and I need to test the PCR on these
sequences. There is only one problem. I don't have these species in
culture so that's why i'm writing this letter. I would like to have DNA
of the next species or groups:
-Bodo caudatus (or any other Kinetoplastida).
-Hexamita sp. (or any other Diplomonadida).
-Tetrahymena sp. (or any other Oligohymenophorea)
-Plasmodium sp. (or any other Haemosporina)
If somebody out there has cultures or DNA isolations of one of these
species I would really like some of it....if they wish to share it.
PLease contact me!
Erik van Hannen
Centre for Limnolgy voice: +31 294 239315
Rijksstraatweg 6 fax : +31 294 232224
3631 AC Nieuwersluis email: vanhannen at cl.nioo.knaw.nl