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Question on mt-DNA

slamovic at rocbi.DNET.roche.com slamovic at rocbi.DNET.roche.com
Fri Feb 2 06:51:06 EST 1996


Hello,

   I'm engaged in a project of phylogeny using cyt-b gene. I'd like to hear
opinions about the best way of amplifying mt-DNA. I see that most people
amplify cyt-b from total DNA, but others isolate the mt-DNA previously in order
to avoid nuclear interferences.
What do you think about this?

Thanks in advance.


Claudio Slamovits
University of Buenos Aires



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